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- substituted enzyme intermediate, in which the enzyme is temporarily modified.
After aspartate binds to the enzyme, the enzyme accepts aspartate's aminogroup to form the substituted enzyme intermediate. The first product, oxaloacetate, subsequently departs. The second substrate, a -ketoglutarate, binds to the enzyme, accepts the amino group from the modified enzyme,
and is then released as the final product, glutamate. In the Cleland
notation, the substrates appear to bounce on and off the enzyme much as a Ping-Pong
ball bounces on a table.
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