Which Biosafety cabinet is usually required when working with biological materials
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The role of the biological safety cabinet can be up to three types of protections: Show
1. Protection of the worker - from harmful elements inside the cabinet 2. Protection of the product inside the cabinet - to explain contamination at work, in an experiment or under guidance 3. Protection of the environment - from various pollutants that are created inside the biological cabinet Types of Biological Safety CabinetThe biological safety cabinet are divided into 3 classes, each group defining the type of protection: Class I biological safety cabinetThe front opening of the cabinet is open, and there is an air flow from the room inside. A lid
with or without gloves can be fitted on the opening.The cabinet protects the worker and the environment only.  Class II biological safety cabinetsThe front opening is open and there is a vertical laminar air flow inside the cabinet.This hood provides protection for the employee, the environment and the product.The principle of protection of this cabinet is in the flow of air from the room to the hood space. Class II itself is divided into 4 groups: A1, A2, B1, B2. A2 B2 Of all of them Class II A2 is the most commonly used. Class III biological safety cabinetSealed glove compartments. Both incoming and outgoing air are filtered through a HEPA filter. There is maximum protection for the employee, the environment and also the sterility of the product. How To Choose BIOLOGICAL CABINETSYou must ask the following questions: 1. What is the type of biological factor according to the risk groups. 2. Is there in addition to the biological risk also a chemical risk or radiation risks. 3. Is it necessary to defend the experiment itself. 4. Is it necessary to limit the release of aerosols generated in the experiment. 5. Are there any special requirements of the workplace. 6.What is the working width required for the cabinet How to work properlyThe work must be planned in advance, and a labeling list of equipment, materials, and order of operations must be prepared -Avoid interfering with the air barrier. -Avoid moving people in the room.
A biological safety cabinet (BSC) is a primary engineering control used to protect personnel against biohazardous or infectious agents and to help maintain quality control of the material being worked with as it filters both the inflow and exhaust air. It is sometimes referred to as a laminar flow or tissue culture hood. These filtered cabinets are primarily designed to protect against exposure to particulates or aerosols. A portion of the air in most BSCs is recirculated back into the lab through its exhaust HEPA filter. This purifies the air of potentially infectious aerosols, animal dander, or both but does not reduce exposure to chemicals. All procedures should be performed in a manner that reduces the generation of aerosolized material and prevents spills. Operations such as centrifugation, vortexing, sonication, and opening containers of infectious materials whose internal pressure may be different from the ambient pressure are known aerosol-generating procedures. These procedures should be conducted inside the BSC or additional measures must be available to mitigate the safety concern. Contact the EHS representative assigned to your BSC or the EHS main line at (734) 647-1143 for guidance regarding proper BSC:
Standard Operating Procedures, Guidelines, and ManualsThe following documents may be required for your research operation, depending on what type of research you are performing, the potential hazards you and your staff will be exposed to, and the engineering controls you are implementing. Some of these documents are in a downloadable “Word” format so that you can print them for your operation as is, or download and modify them to meet your unique needs. When you click the link it will automatically go to the download file.
EHS Engineering Controls of Standard Care
Lab Director ResponsibilitiesLab directors (faculty/lab managers/supervisors) must:
Lab Personnel ResponsibilitiesLab personnel must:
CertificationBiological safety cabinets must be inspected and certified by EHS:
If your BSC has not been certified within the last year, or if you have any questions regarding BSCs, call EHS at (734) 647-1143. What does the Class II biosafety cabinet protect?What type of protection does Class II BSC offer? Class II Biosafety Cabinets are open-fronted which protect the laboratory workers and the environment from harmful biological agents. Class II BSCs also prevent biological materials (i.e cell cultures, microbiological stocks) inside it from being contaminated.
What is a Class 1 biosafety cabinet?A Class I cabinet is defined as a ventilated cabinet for personnel and environmental protection. Class I cabinets do not offer product protection from contamination, significantly limiting their applications. They use unrecirculated airflow away from the operator.
What is class II B2 biosafety cabinet?Purifier Logic Class II, Type B2 Biosafety Cabinets provide personnel, product and environmental protection from hazardous particulates such as agents that require Biosafety Level 1, 2 or 3 containment.
What are the 3 biological safety cabinets?There are three classes of BSCs: Class I, II and III. While all three classes afford personnel and environmental protection, only Class II and III cabinets provide product protection. Class I BSCs are suitable for work involving low to moderate-risk agents.
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